Issue 8, 2012
From the journal:

Chemical Communications

Direct characterization of protein oligomers and their quaternary structures by single-molecule FRET†

Cheolhee Kim,a   Jae Yeol Kim,a   Seung Hyeon Kim,a   Byung Il Leeb  and  Nam Ki Lee*a  

* Corresponding authors

a Department of Physics and School of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang 790–784, South Korea
E-mail: nklee@postech.ac.kr

b National Cancer Center, Goyang 410-769, South Korea

Abstract

Using a single-molecule method, we directly distinguish among oligomers from monomers to tetramers and determine their quaternary structures. Using this method, we found that RecR forms a stable dimer and its oligomeric form is modulated by its own concentration and the interaction with RecO.

Graphical abstract: Direct characterization of protein oligomers and their quaternary structures by single-molecule FRET

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Article information

DOI
https://doi.org/10.1039/C2CC16528G
Article type
Communication
Submitted
20 Oct 2011
Accepted
23 Nov 2011
First published
24 Nov 2011

Chem. Commun., 2012,48, 1138-1140

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Direct characterization of protein oligomers and their quaternary structures by single-molecule FRET

C. Kim, J. Y. Kim, S. H. Kim, B. I. Lee and N. K. Lee, Chem. Commun., 2012, 48, 1138 DOI: 10.1039/C2CC16528G

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