Issue 8, 2011
From the journal:

Chemical Communications

Construction of a thiamin sensor from the periplasmic thiamin binding protein†

Jeremiah W. Hanes,a   Debashree Chatterjee,a   Erika V. Soriano,a   Steven E. Ealicka  and  Tadhg P. Begley*b  

* Corresponding authors

a Department of Chemistry and Chemical Biology, Cornell University, Ithaca, USA

b Department of Chemistry, Interdisciplinary Life Sciences Building, 3474 TAMU, Texas A&M University, College Station, Texas 77843, USA
E-mail: begley@chem.tamu.edu
Fax: +1 979-458-5735
Tel: +1 979-862-4091

Abstract

This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(l-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.

Graphical abstract: Construction of a thiamin sensor from the periplasmic thiamin binding protein

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Article information

DOI
https://doi.org/10.1039/C0CC04228E
Article type
Communication
Submitted
05 Oct 2010
Accepted
10 Nov 2010
First published
02 Dec 2010

Chem. Commun., 2011,47, 2273-2275

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Construction of a thiamin sensor from the periplasmic thiamin binding protein

J. W. Hanes, D. Chatterjee, E. V. Soriano, S. E. Ealick and T. P. Begley, Chem. Commun., 2011, 47, 2273 DOI: 10.1039/C0CC04228E

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