Issue 43, 2010
From the journal:

Chemical Communications

Sandwich probes: two simultaneous reactions for templated nucleic acid detection†

Daniel J. Kleinbauma  and  Eric T. Kool*a  

* Corresponding authors

a Department of Chemistry, Stanford University, Stanford, USA
E-mail: kool@stanford.edu
Fax: +1 650-725-0259
Tel: +1 650-724-4741

Abstract

Fluorescence-quenched nucleic acid probes with reactive moieties at both the 5′ and 3′ ends are synthesized and tested for reaction with two adjacent nucleophile-containing DNAs. These probes improve signal to background over singly reactive probes and can discriminate single nucleotide polymorphisms in the target DNA or RNA.

Graphical abstract: Sandwich probes: two simultaneous reactions for templated nucleic acid detection

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Article information

DOI
https://doi.org/10.1039/C0CC01968B
Article type
Communication
Submitted
19 Jun 2010
Accepted
14 Sep 2010
First published
07 Oct 2010

Chem. Commun., 2010,46, 8154-8156

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Sandwich probes: two simultaneous reactions for templated nucleic acid detection

D. J. Kleinbaum and E. T. Kool, Chem. Commun., 2010, 46, 8154 DOI: 10.1039/C0CC01968B

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