Jump to main content
Jump to site search

Issue 3, 2010
Previous Article Next Article

FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

Author affiliations

Abstract

We report a stepwise FLAG-tagging strategy for the purification of target proteins for bioactive metabolites. This method realizes the microscale purification and identification of target protein from as few as 1 × 105 differentiated cells. Using this method, we isolated and identified MetE as a cytosolic target protein of potassium isolespedezate, a metabolite controlling plant nyctinasty.

Graphical abstract: FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Aug 2009, accepted on 23 Oct 2009 and first published on 16 Nov 2009


Article type: Communication
DOI: 10.1039/B915843J
Citation: Chem. Commun., 2010,46, 469-471
  •   Request permissions

    FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

    Y. Manabe, M. Mukai, S. Ito, N. Kato and M. Ueda, Chem. Commun., 2010, 46, 469
    DOI: 10.1039/B915843J

Search articles by author

Spotlight

Advertisements