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FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

Yoshiyuki Manabe,a   Makoto Mukai,a   Satoko Ito,a   Nobuki Katoa and   Minoru Ueda*a  
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a
Department of Chemistry, Tohoku University, 6-3 Aramakiaza-aoba, Aoba-ku, Sendai 980-8578, Japan
E-mail: ueda@mail.tains.tohoku.ac.jp;
Fax: (+) 81-22-795-6553 ;
Tel: (+) 81-22-795-6553
Chem. Commun., 2010,46, 469-471

DOI: 10.1039/B915843J
Received 03 Aug 2009, Accepted 23 Oct 2009
First published online 16 Nov 2009
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