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Issue 29, 2008
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Specific N-terminal protein labelling: use of FMDV 3Cproprotease and native chemical ligation

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Abstract

We report an effective strategy for generating N-terminal cysteinyl proteins by proteolytic cleavage using the enzyme 3Cpro, suitable for a wide range of applications via native chemical ligation.

Graphical abstract: Specific N-terminal protein labelling: use of FMDV 3Cproprotease and native chemical ligation

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Publication details

The article was received on 21 Apr 2008, accepted on 14 May 2008 and first published on 25 Jun 2008


Article type: Communication
DOI: 10.1039/B806727A
Citation: Chem. Commun., 2008, 3369-3371
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    Specific N-terminal protein labelling: use of FMDV 3Cproprotease and native chemical ligation

    G. K. Busch, E. W. Tate, P. R. J. Gaffney, E. Rosivatz, R. Woscholski and R. J. Leatherbarrow, Chem. Commun., 2008, 3369
    DOI: 10.1039/B806727A

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