Issue 43, 2007
From the journal:

Chemical Communications

Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays†

Md Monsur Ali,ab   Shunxing Su,c   Carlos D. M. Filipe,c   Robert Peltonc  and  Yingfu Li*ab  

* Corresponding authors

a Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, ON, Canada
E-mail: liying@mcmaster.ca
Fax: (+1) 905-522-9033

b Department of Chemistry, McMaster University, 1200 Main St. W., Hamilton, ON, Canada

c Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada

Abstract

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA–microgel conjugates can be explored for the development of DNA based bioassays and biosensors.

Graphical abstract: Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays

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Article information

DOI
https://doi.org/10.1039/B709817K
Article type
Communication
Submitted
27 Jun 2007
Accepted
08 Aug 2007
First published
22 Aug 2007

Chem. Commun., 2007, 4459-4461

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Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays

M. M. Ali, S. Su, C. D. M. Filipe, R. Pelton and Y. Li, Chem. Commun., 2007, 4459 DOI: 10.1039/B709817K

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