Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip

Wenzhang Chen; Xuefeng Yin; Jinxia Mu; Yan Yin

doi:10.1039/B700200A

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Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip†

Wenzhang Chen,^a Xuefeng Yin,*^a Jinxia Mu^a and Yan Yin^b

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* Corresponding authors

^a Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, China
E-mail: yinxf@zju.edu.cn
Fax: +86-571-87952070
Tel: +86-571-87952070

^b Chemistry Department, University of New Hampshire, 23 College Road, Durham, NH, USA

Abstract

A novel microfluidic chip based Edman degradation system is developed, in which Edman degradation can be carried out with peptide at a subfemtomole level; combined with MALDI-TOF-MS detection, the identification specificity of gel-separated protein in low abundance has been drastically improved.

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Supplementary files

Table S1: The peptide sequences matched to the tryptic peptide mass fingerprint of Enolase 1. Table S2: The peptide sequences matched to the tryptic peptide mass fingerprint of T-cell receptor beta chain PDF (110K)

Article information

DOI: https://doi.org/10.1039/B700200A
Article type: Communication
Submitted: 08 Jan 2007
Accepted: 08 Mar 2007
First published: 27 Mar 2007

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Chem. Commun., 2007, 2488-2490

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Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip

W. Chen, X. Yin, J. Mu and Y. Yin, Chem. Commun., 2007, 2488 DOI: 10.1039/B700200A

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Chemical Communications

Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip†

Abstract

Supplementary files

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Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip

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