Are branched chain fatty acids the natural substrates for P450BM3?

Max J. Cryle; Rocio D. Espinoza; Sarah J. Smith; Nicholas J. Matovic; James J. De Voss

doi:10.1039/B601202G

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Are branched chain fatty acids the natural substrates for P450_BM3?†

Max J. Cryle,^a Rocio D. Espinoza,^a Sarah J. Smith,^a Nicholas J. Matovic^a and James J. De Voss*^a

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* Corresponding authors

^a Department of Chemistry, University of Queensland, St. Lucia, Brisbane, Australia
E-mail: j.devoss@uq.edu.au
Fax: 61 7 3365 4299
Tel: 61 7 3365 3825

Abstract

Branched chain fatty acids are substrates for cytochrome P450_BM3 (CYP102) from Bacillus megaterium; oxidation of C₁₅ and C₁₇iso and anteiso fatty acids by P450_BM3 leads to the formation of hydroxylated products that possess high levels of regiochemical and stereochemical purity.

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Supplementary files

Conditions of turnover and GC analysis for 1– 4; enantiomeric HPLC analysis of turnover of 1 and 3; characterisation and MS fragmentation and response factors for 5a– 5c + 6a– 6c; binding constant and rate determination for 1– 4 PDF (181K)

Article information

DOI: https://doi.org/10.1039/B601202G
Article type: Communication
Submitted: 26 Jan 2006
Accepted: 16 Mar 2006
First published: 30 Mar 2006

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Chem. Commun., 2006, 2353-2355

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Are branched chain fatty acids the natural substrates for P450_BM3?

M. J. Cryle, R. D. Espinoza, S. J. Smith, N. J. Matovic and J. J. De Voss, Chem. Commun., 2006, 2353 DOI: 10.1039/B601202G

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