68Zn isotope exchange experiments reveal an unusual kinetic lability of the metal ions in the di-zinc form of IMP-1 metallo-β-lactamase

Stefan Siemann; Hamid R. Badiei; Vassili Karanassios; Thammaiah Viswanatha; Gary I. Dmitrienko

doi:10.1039/B514227J

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⁶⁸Zn isotope exchange experiments reveal an unusual kinetic lability of the metal ions in the di-zinc form of IMP-1 metallo-β-lactamase†

Stefan Siemann,^a Hamid R. Badiei,^b Vassili Karanassios,^b Thammaiah Viswanatha^b and Gary I. Dmitrienko*^b

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* Corresponding authors

^a Department of Chemistry and Biochemistry, Laurentian University, 935 Ramsey Lake Rd., Sudbury, Ontario, Canada
E-mail: ssiemann@laurentian.ca
Fax: +1 (705) 675 4844
Tel: +1 (705) 675 1151

^b Department of Chemistry, University of Waterloo, 200 University Ave. W, Waterloo, Ontario, Canada
E-mail: dmitrien@uwaterloo.ca
Fax: +1 (519) 746-0435
Tel: +1 (519) 888 4642

Abstract

The apparently paradoxical behaviour of facile exchange (kinetic lability) of tightly bound (thermodynamic stability) zinc ions in the enzyme IMP-1 metallo-β-lactamase with Zn-68 and cadmium ions, as indicated by in-torch vaporization inductively-coupled plasma mass spectrometry (ITV-ICP-MS) and electrospray-ionization mass spectrometry (ESI-MS), is consistent with the involvement of a third metal ion in promoting Lewis acid/base type exchange processes.

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Supplementary files

Experimental details, tables of isotope abundances, ITV-ICP-MS diagram and operating conditions, conversion of ⁶⁸Zn/ ⁶⁴Zn isotope ratios into ttr values, time-dependence of measured isotope ratios, ESI-MS spectra PDF (602K)

Article information

DOI: https://doi.org/10.1039/B514227J
Article type: Communication
Submitted: 07 Oct 2005
Accepted: 18 Nov 2005
First published: 14 Dec 2005

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Chem. Commun., 2006, 532-534

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⁶⁸Zn isotope exchange experiments reveal an unusual kinetic lability of the metal ions in the di-zinc form of IMP-1 metallo-β-lactamase

S. Siemann, H. R. Badiei, V. Karanassios, T. Viswanatha and G. I. Dmitrienko, Chem. Commun., 2006, 532 DOI: 10.1039/B514227J

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