Design and synthesis of artificial phospholipid for selective cleavage of integral membrane protein

Takumi Furuta; Minatsu Sakai; Hiroyasu Hayashi; Tomohiro Asakawa; Fumi Kataoka; Satoshi Fujii; Takashi Suzuki; Yasuo Suzuki; Kiyoshi Tanaka; Nathan Fishkin; Koji Nakanishi

doi:10.1039/B507917A

Design and synthesis of artificial phospholipid for selective cleavage of integral membrane protein†

Takumi Furuta,*^a Minatsu Sakai,^a Hiroyasu Hayashi,^a Tomohiro Asakawa,^a Fumi Kataoka,^b Satoshi Fujii,^b Takashi Suzuki,^c Yasuo Suzuki,^c Kiyoshi Tanaka,‡^a Nathan Fishkin^d and Koji Nakanishi*^d

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* Corresponding authors

^a Department of Synthetic Organic & Medicinal Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka, Japan
E-mail: furuta@u-shizuoka-ken.ac.jp
Fax: +81-54-264-5745
Tel: +81-54-264-5742

^b Department of Physical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka, Japan

^c Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, CREST, JST, COE Program in the 21st Century, 52-1 Yada, Shizuoka, Japan

^d Department of Chemistry, Columbia University, New York, USA
E-mail: kn5@columbia.edu
Fax: +1-212-932-8273
Tel: +1-212-854-2169

Abstract

An artificial phospholipid, possessing saturated alkyl chains as a membrane anchor and protein recognition site as well as an Fe(III)–EDTA moiety as a protein cleavable polar head group, was designed and synthesized based on the amidite method for the purpose of examination of cleavage of integral membrane proteins.

Chemical Communications

Design and synthesis of artificial phospholipid for selective cleavage of integral membrane protein†

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Design and synthesis of artificial phospholipid for selective cleavage of integral membrane protein

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