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The formation constants of the ligandN,N,N′,N′-tetrakis(carbamoylmethyl)-ethylenediamine suggest that the amide oxygen is a stronger Lewis base in water than the alcoholic oxygen, or water, and that part of the selectivity for Ca2+ over Mg2+ shown by calcium-binding proteins such as calmodulin or annexin may be due to the higher affinity of Ca2+ for the O-donor of the Ca-binding amide groups present in such proteins.
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