SCHEDULED MAINTENANCE
The regioselectivity of the catalytic hydroxylation of phenylcyclohexane 1 by cytochrome P450cam can be altered dramatically by site-directed mutagenesis: the Y96F single site mutant gives 81%cis-3-phenylcyclohexanol 2, with 22% enantiomeric excess (ee), while the Y96F–V247A double mutant gives 97% 2 with 42% ee and the Y96F–V247L double mutant gives 83%trans-4-phenylcyclohexanol 4.
N. E. Jones, P. A. England, D. A. Rouch and L. Wong, Chem. Commun., 1996, 2413 DOI: 10.1039/CC9960002413
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