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Issue 10, 2014
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Cellulose-based polyelectrolyte complex nanoparticles for DNA vaccine delivery

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Abstract

Cellulose-based nanoparticles were prepared from oppositely charged carboxymethyl cellulose (CMC) and quaternized cellulose (QC) in an aqueous medium. The DNA complexing capacity of CMC–QC nanoparticles and the transfection efficiency of the DNA-loaded nanoparticles in COS-7 cells were first investigated using pEGFP-N1—a plasmid DNA encoding the enhanced green fluorescent protein (EGFP)—as a model, and then with a candidate DNA vaccine, pMASIA-tPAs-tE2.2, that has been developed against infection with bovine viral diarrhea virus (BVDV). The results revealed that CMC–QC nanoparticles could bind DNA efficiently, and the optimized DNA-loaded nanoparticles mediated very effective transfection in COS-7 cells, which was comparable to that achieved with Lipofectamine 2000. The novel CMC–QC nanoparticles show promise as a delivery system for DNA vaccines.

Graphical abstract: Cellulose-based polyelectrolyte complex nanoparticles for DNA vaccine delivery

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Publication details

The article was received on 04 Jun 2014, accepted on 20 Jun 2014 and first published on 07 Jul 2014


Article type: Paper
DOI: 10.1039/C4BM00202D
Citation: Biomater. Sci., 2014,2, 1440-1449
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    Cellulose-based polyelectrolyte complex nanoparticles for DNA vaccine delivery

    Y. Song, Y. Zhou, S. van Drunen Littel-van den Hurk and L. Chen, Biomater. Sci., 2014, 2, 1440
    DOI: 10.1039/C4BM00202D

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