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Issue 3, 2014
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A new method to cross-link a polyplex for enhancing in vivo stability and transfection efficiency

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Abstract

Disulfide-exchange was found to cross-link the polyplex of disulfide-containing poly(amido amine) and pDNA with heating of the polyplex solution over a short time. The cross-linked polyplexes based on disulfide-containing poly(amido amine) have excellent stability under physiological salt conditions, and have significantly enhanced transfection activity in the serum media compared to non-cross-linked polyplexes. In vivo, ICR mice were injected with the polyplex through the tail vein, the results show that the transfection efficiency of the cross-linked polyplex is higher than that of the non-cross-linked variety. Furthermore, the polyplex containing Cy5 labelled DNA was also injected into the mice to illustrate the stability and distribution of the polyplex, cross-linked polyplexes show a much brighter luminescence than the non-cross-linked ones. This method does not need a cross-linker or catalyst, and there are no impurities produced, it may be an elegant approach to resolve the dilemma of in vivo application of a DNA polyplex, with excellent stability whilst in circulation and a rapid unpacking of the polyplex inside the cells.

Graphical abstract: A new method to cross-link a polyplex for enhancing in vivo stability and transfection efficiency

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Publication details

The article was received on 30 Aug 2013, accepted on 18 Oct 2013 and first published on 27 Nov 2013


Article type: Paper
DOI: 10.1039/C3BM60204D
Citation: Biomater. Sci., 2014,2, 390-398
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    A new method to cross-link a polyplex for enhancing in vivo stability and transfection efficiency

    J. Piao, J. Yan, M. Wang, D. Wu and Y. You, Biomater. Sci., 2014, 2, 390
    DOI: 10.1039/C3BM60204D

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