Issue 25, 2018

A low-cost integrated biosensing platform based on SiN nanophotonics for biomarker detection in urine

Abstract

We present a low-cost integrated nanophotonic lab-on-a-chip platform suitable for point-of-care (POC) biomarker analysis. The sensor chip included in the platform contains multiplexed Mach–Zehnder interferometers with an on-chip optical spectral analyser consisting of an arrayed-waveguide grating. The sensor chip is fabricated in silicon nitride material, which makes it compatible with consumer-electronics-grade sources and detectors, leading to the possibility of low-cost instrumentation. The nanophotonic sensor chip exhibits a detection limit of 6 × 10−6 RIU (Refractive Index Units), which is in the same order of magnitude as the reported values for state-of-the-art evanescent wave sensors. The sensor chip is biofunctionalised with specific bioreceptors and integrated into a polymer microfluidic cartridge. The POC instrumentation platform contains optical excitation and read-out sub-systems and dedicated on-board software for real-time analysis of patient samples. To demonstrate the versatility of the platform, we present results both on the detection of an antigen related to tuberculosis directly in urine samples using a laboratory prototype and on the detection of a protein biomarker (CRP) related to inflammation using the integrated instrument.

Graphical abstract: A low-cost integrated biosensing platform based on SiN nanophotonics for biomarker detection in urine

Article information

Article type
Paper
Submitted
26 Mar 2018
Accepted
04 Jun 2018
First published
06 Jun 2018

Anal. Methods, 2018,10, 3066-3073

A low-cost integrated biosensing platform based on SiN nanophotonics for biomarker detection in urine

D. Martens, P. Ramirez-Priego, M. S. Murib, A. A. Elamin, A. B. Gonzalez-Guerrero, M. Stehr, F. Jonas, B. Anton, N. Hlawatsch, P. Soetaert, R. Vos, A. Stassen, S. Severi, W. Van Roy, R. Bockstaele, H. Becker, M. Singh, L. M. Lechuga and P. Bienstman, Anal. Methods, 2018, 10, 3066 DOI: 10.1039/C8AY00666K

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