Jump to main content
Jump to site search

Issue 14, 2018
Previous Article Next Article

Multiplexed microRNA expression profiling by combined asymmetric PCR and label-free detection using silicon photonic sensor arrays

Author affiliations

Abstract

Analysis methods based upon the quantitative, real-time polymerase chain reaction are extremely powerful; however, they face intrinsic limitations in terms of target multiplexing. In contrast, silicon photonic microring resonators represent a modularly multiplexable sensor array technology that is well-suited to the analysis of targeted biomarker panels. In this manuscript we employ an asymmetric polymerase chain reaction approach to selectively amplify copies of cDNAs generated from targeted miRNAs before multiplexed, label-free quantitation through hybridization to microring resonator arrays pre-functionalized with capture sequences. This method, which shows applicability to low input amounts and a large dynamic range, was demonstrated for the simultaneous detection of eight microRNA targets from twenty primary brain tumor samples with expression profiles in good agreement with literature precedent.

Graphical abstract: Multiplexed microRNA expression profiling by combined asymmetric PCR and label-free detection using silicon photonic sensor arrays

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Jan 2018, accepted on 15 Mar 2018 and first published on 23 Mar 2018


Article type: Paper
DOI: 10.1039/C8AY00190A
Citation: Anal. Methods, 2018,10, 1618-1623
  •   Request permissions

    Multiplexed microRNA expression profiling by combined asymmetric PCR and label-free detection using silicon photonic sensor arrays

    R. M. Graybill, M. C. Cardenosa-Rubio, H. Yang, M. D. Johnson and R. C. Bailey, Anal. Methods, 2018, 10, 1618
    DOI: 10.1039/C8AY00190A

Search articles by author

Spotlight

Advertisements