Issue 45, 2017

A one-step rapid screening test of Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay

Abstract

A rapid and specific, hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection of Listeria monocytogenes in food and food products, using a real-time turbidimeter platform (LAMP-turbidity). The principle behind this method relies on an increase in a DNA yield, which correlates with the production of magnesium pyrophosphate, and the results can be determined via an amplification curve within 1 h. The specificity test revealed that L. monocytogenes (DMST 17303) was observed from 34.1 to 38.3 min, while thirty strains of non-L. monocytogenes demonstrated no cross-reactions. The limits of detection for purified genomic DNA and pure culture were 800 pg μL−1 and 2.82 × 103 CFU mL−1, respectively. Investigation on 200 raw chicken meat samples indicated that the specificity, sensitivity, and accuracy of LAMP-turbidity were 100%, 62.75%, and 90.50%, respectively. These data suggest that an hly-based, real-time, quantitative LAMP-turbidity assay can be an applicable tool for the epidemiological screening of L. monocytogenes in food and food products.

Graphical abstract: A one-step rapid screening test of Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay

Article information

Article type
Paper
Submitted
18 Jul 2017
Accepted
17 Oct 2017
First published
09 Nov 2017
This article is Open Access
Creative Commons BY license

Anal. Methods, 2017,9, 6403-6410

A one-step rapid screening test of Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay

S. Wachiralurpan, T. Sriyapai, S. Areekit, P. Sriyapai, D. Thongphueak, S. Santiwatanakul and K. Chansiri, Anal. Methods, 2017, 9, 6403 DOI: 10.1039/C7AY01750B

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