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Issue 22, 2014
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Development of a sensitive and specific enzyme-linked immunosorbent assay for the determination of fludioxonil residues in fruit juices

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Abstract

Fludioxonil is a fungicide with a singular mode of action that is widely employed in the treatment of fruit crops. A competitive enzyme-linked immunosorbent assay (cELISA) has been developed and validated for the determination of residues of this fungicide in fruit juices. A collection of monoclonal antibodies (mAbs) against the fungicide fludioxonil has been produced and characterized using direct and indirect cELISAs. The high affinity achieved (IC50 lower than 0.5 μg L−1) considerably improved that obtained with previous polyclonal antibodies. The proposed cELISA was applied to the analysis of fludioxonil residues in apple juice and red grape must samples with a limit of quantification of 5 and 10 μg L−1, respectively. A validation of the method has been performed in terms of sensitivity, selectivity, trueness, precision, and robustness. Finally, blind-spiked samples were measured by the proposed cELISA and by a gas chromatography-mass spectrometry (GC-MS) method, with equivalent results by both methodologies.

Graphical abstract: Development of a sensitive and specific enzyme-linked immunosorbent assay for the determination of fludioxonil residues in fruit juices

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Publication details

The article was received on 25 Jul 2014, accepted on 11 Sep 2014 and first published on 15 Sep 2014


Article type: Paper
DOI: 10.1039/C4AY01756K
Citation: Anal. Methods, 2014,6, 8924-8929
  • Open access: Creative Commons BY license
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    Development of a sensitive and specific enzyme-linked immunosorbent assay for the determination of fludioxonil residues in fruit juices

    J. V. Mercader, A. Abad-Fuentes, C. Agulló, A. Abad-Somovilla and F. A. Esteve-Turrillas, Anal. Methods, 2014, 6, 8924
    DOI: 10.1039/C4AY01756K

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