Specific antibody-induced fluorescence quenching for the development of a directly applicable and label-free immunoassay

Abstract

A simple, directly applicable and label-free immunoassay was proposed based on a specific immune binding reaction induced fluorescence quenching of an analyte. Aflatoxin B₁ (AFB₁) was taken as the model analyte, whose intrinsic fluorescence was quenched by the specific anti-AFB₁ monoclonal antibody. This immunoreaction-induced fluorescence quenching was utilized to quantitatively determine AFB₁.