Issue 5, 2014

Facile functionalization of Ag@SiO2 core–shell metal enhanced fluorescence nanoparticles for cell labeling

Abstract

We describe a versatile approach for functionalizing core–shell Ag@SiO2 nanoparticles for live-cell imaging. The approach uses physical adsorption and does not need covalent linkage to synthesize antibody-based labels. The surface orientation is not controlled in this approach, but the signal enhancement is strong and consistent. Antibodies were then attached using a non-covalent process that takes advantage of biotin–avidin affinity. Metal-enhanced nanoparticles doped with rhodamine B were used as the luminescent reporter. The enhancement of rhodamine B was between 2.7 and 6.8 times. We demonstrated labeling of CD19+ Ramos B lymphocytes and CD4+ HuT 78 T lymphocytes using anti-CD19 and anti-CD4 nanocomposite labels, respectively. This physical adsorption process can accommodate a variety of fluorophore types, and has broad potential in bioanalytical and biosensing applications.

Graphical abstract: Facile functionalization of Ag@SiO2 core–shell metal enhanced fluorescence nanoparticles for cell labeling

Article information

Article type
Technical Note
Submitted
02 Dec 2013
Accepted
10 Jan 2014
First published
14 Jan 2014

Anal. Methods, 2014,6, 1598-1602

Facile functionalization of Ag@SiO2 core–shell metal enhanced fluorescence nanoparticles for cell labeling

M. Dong, Y. Tian and D. Pappas, Anal. Methods, 2014, 6, 1598 DOI: 10.1039/C3AY42150C

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