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Issue 23, 2013
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Determination of 2-methoxyestradiol from endometrial cancer patients' urine by LC-MS/MS with hollow fiber liquid-phase microextraction

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Abstract

2-Methoxyestradiol (2-MeOE2) is a potent antiangiogenic and antitumor agent in preclinical models due to its antiproliferative and apoptotic actions. In endometrial cancer patients' urine, the content of 2-MeOE2 may be less than that in urine samples of healthy people. The sensitivity can be elevated to a high level by using hollow fiber liquid-phase microextraction (HF-LPME) as an enriched pretreatment method with LC-MS/MS as a detection means. In the experimental section, the urine sample was firstly handled with sodium hydroxide for hydrolysis and then extracted with HF-LPME. After that the extracts were derivatised and finally detected with LC-MS/MS. For HF-LPME, we obtained an enrichment factor of 180 along with a good sample clean-up. The calibration curve was linear with a linear regression correlation coefficient typically greater than 0.996. The limit of detection (LOD) for 2-MeOE2 was 0.14 pg mL−1 with good repeatability and reproducibility. The proposed method was applied for the analysis of human urine samples from endometrial cancer patients and healthy people.

Graphical abstract: Determination of 2-methoxyestradiol from endometrial cancer patients' urine by LC-MS/MS with hollow fiber liquid-phase microextraction

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Publication details

The article was received on 04 Jul 2013, accepted on 01 Sep 2013 and first published on 03 Sep 2013


Article type: Paper
DOI: 10.1039/C3AY41105B
Citation: Anal. Methods, 2013,5, 6670-6676
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    Determination of 2-methoxyestradiol from endometrial cancer patients' urine by LC-MS/MS with hollow fiber liquid-phase microextraction

    Y. Liu, L. Li, M. Xu, X. Jiang and Y. Jiang, Anal. Methods, 2013, 5, 6670
    DOI: 10.1039/C3AY41105B

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