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Issue 11, 2013
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Proteinpolyphenol interaction on silica beads for astringency tests based on eye, photography or reflectance detection modes

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Abstract

Astringency is an organoleptic property of beverages and food products resulting mainly from the interaction of salivary proteins with dietary polyphenols. It is of great importance to consumers, but the only effective way of measuring it involves trained sensorial panellists, providing subjective and expensive responses. Concurrent chemical evaluations try to screen food astringency, by means of polyphenol and protein precipitation procedures, but these are far from the real human astringency sensation where not all polyphenolprotein interactions lead to the occurrence of precipitate. Here, a novel chemical approach that tries to mimic proteinpolyphenol interactions in the mouth is presented to evaluate astringency. A protein, acting as a salivary protein, is attached to a solid support to which the polyphenol binds (just as happens when drinking wine), with subsequent colour alteration that is fully independent from the occurrence of precipitate. Employing this simple concept, Bovine Serum Albumin (BSA) was selected as the model salivary protein and used to cover the surface of silica beads. Tannic Acid (TA), employed as the model polyphenol, was allowed to interact with the BSA on the silica support and its adsorption to the protein was detected by reaction with Fe(III) and subsequent colour development. Quantitative data of TA in the samples were extracted by colorimetric or reflectance studies over the solid materials. The analysis was done by taking a regular picture with a digital camera, opening the image file in common software and extracting the colour coordinates from HSL (Hue, Saturation, Lightness) and RGB (Red, Green, Blue) colour model systems; linear ranges were observed from 10.6 to 106.0 μmol L−1. The latter was based on the Kubelka–Munk response, showing a linear gain with concentrations from 0.3 to 10.5 μmol L−1. In either of these two approaches, semi-quantitative estimation of TA was enabled by direct eye comparison. The correlation between the levels of adsorbed TA and the astringency of beverages was tested by using the assay to check the astringency of wines and comparing these to the response of sensorial panellists. Results of the two methods correlated well. The proposed sensor has significant potential as a robust tool for the quantitative/semi-quantitative evaluation of astringency in wine.

Graphical abstract: Protein–polyphenol interaction on silica beads for astringency tests based on eye, photography or reflectance detection modes

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Publication details

The article was received on 30 Nov 2012, accepted on 04 Apr 2013 and first published on 26 Apr 2013


Article type: Paper
DOI: 10.1039/C3AY26478E
Citation: Anal. Methods, 2013,5, 2694-2703
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    Proteinpolyphenol interaction on silica beads for astringency tests based on eye, photography or reflectance detection modes

    J. R. L. Guerreiro, D. S. Sutherland, V. De Freitas and M. G. F. Sales, Anal. Methods, 2013, 5, 2694
    DOI: 10.1039/C3AY26478E

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