Issue 4, 2013

A novel method of chemiluminescence detection coupled with high-performance liquid chromatography and its application in direct determination of tartaric, malic and citric acids in fruit juice

Abstract

In the present work, a new method of chemiluminescence detection coupled with high performance liquid chromatography for three organic acids—tartaric, malic and citric acids is described. The detection is based on the enhancement of the organic acids for the chemiluminescence reaction between cerous sulfate (Ce(SO4)2) and tris (2,2′-bipyridyl)ruthenium(II) (Ru(bipy)32+). The latter was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent. Under the optimal conditions, the linear ranges were 7.5 × 10−7 g mL−1 to 7 × 10−6 g mL−1 for tartaric acid, 4.0 × 10−7 g mL−1 to 1.0 × 10−5 g mL−1 for malic acid and 1 × 10−7 g mL−1 to 1.5 × 10−6 g mL−1 for citric acid, and the detection limits were 1.5 × 10−7 g mL−1, 2.0 × 10−7 g mL−1, 4.0 × 10−8 g mL−1 for tartaric, malic and citric acid, respectively. The proposed method had been successfully applied to the determination of three organic acids in fruit juice – with only filtration and dilution steps.

Graphical abstract: A novel method of chemiluminescence detection coupled with high-performance liquid chromatography and its application in direct determination of tartaric, malic and citric acids in fruit juice

Supplementary files

Article information

Article type
Paper
Submitted
03 Sep 2012
Accepted
26 Nov 2012
First published
28 Nov 2012

Anal. Methods, 2013,5, 982-986

A novel method of chemiluminescence detection coupled with high-performance liquid chromatography and its application in direct determination of tartaric, malic and citric acids in fruit juice

M. Deng, F. Chen and X. Long, Anal. Methods, 2013, 5, 982 DOI: 10.1039/C2AY25974E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements