Issue 2, 2013

Dispersive solid phase extraction coupled with HPLC-UV for simultaneous determination of chlorpyrifos and 3,5,6-trichloro-2-pyridinol in soil samples

Abstract

A simple method was proposed for simultaneous determination of chlorpyrifos and its hydrolysis and biodegradation product 3,5,6-trichloro-2-pyridinol (TCP) in soil samples. The analytes were extracted with ethyl acetate (EtOAc) under optimized pretreatment conditions such as the type, volume and pH of extraction solvent. Clean-up of the extract from soil was conducted by dispersive solid phase extraction (DSPE) using activated carbon as the adsorbent. High performance liquid chromatography with ultraviolet detection (HPLC-UV) was used for simultaneous determination of the two analytes. Under the optimized HPLC conditions, the limits of detection (LOD), obtained on signal-to-noise ratio (S/N) of 3, for chlorpyrifos and TCP were 0.0080 mg kg−1 and 0.0120 mg kg−1, respectively. Linear calibration curves were obtained in the range of 0.05–2.00 mg kg−1 and 0.03–2.00 mg kg−1 for chlorpyrifos and TCP, respectively. The spike recoveries from soil ranged from 89.4% to 114%, and the relative standard deviation (RSD) was in the range of 0.80%–14%.

Graphical abstract: Dispersive solid phase extraction coupled with HPLC-UV for simultaneous determination of chlorpyrifos and 3,5,6-trichloro-2-pyridinol in soil samples

Article information

Article type
Paper
Submitted
02 Sep 2012
Accepted
07 Nov 2012
First published
14 Nov 2012

Anal. Methods, 2013,5, 536-540

Dispersive solid phase extraction coupled with HPLC-UV for simultaneous determination of chlorpyrifos and 3,5,6-trichloro-2-pyridinol in soil samples

J. Xiong, X. Tang, G. Zhou, Z. Guan and L. Wu, Anal. Methods, 2013, 5, 536 DOI: 10.1039/C2AY25972A

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