A fluorescence-based method for the characterization of amino loading density on the flat surface of functionalized glass tubes

Abstract

In this study, a fluorescence-based method was used to characterize the amino loading density on the flat surface of glass tubes functionalized by silylation with (3-aminopropyl)trimethoxysilane and then fluorescence-labeled with fluorescein isothiocyanate. The immobilized fluorescent molecules on the surface of the glass tubes were released by treatment with hydrofluoric acid. The fluorescence of the released solution was detected by a multifunction micro-plate reader, and the concentration of the fluorescent molecules was calculated from a calibration curve based on the reference compound 2a. A density calculation formula D = 27.95Y/S was developed, where Y represents the fluorescence intensity of the solutions and S represents the surface area of the glass tubes.