Issue 9, 2011
From the journal:

Analytical Methods

Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis†

Kris P. F. Janssen,a   Karel Knez,a   Jeroen Pollet,a   Scott J. Roberts,b   Jan Schrootenc  and  Jeroen Lammertyn*a  

* Corresponding authors

a BIOSYST—MeBioS, Katholieke Universiteit Leuven, Willem De Croylaan 42, Leuven, Belgium
E-mail: jeroen.lammertyn@biw.kuleuven.be
Fax: +3216322955
Tel: +3216321459

b Laboratory for Skeletal Development and Joint Disorders, Katholieke Universiteit Leuven, O&N 1, Herestraat 49, PB813, Leuven, Belgium
E-mail: scott.roberts@med.kuleuven.be

c Department of Metallurgy and Materials Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 44, PB2450, Leuven, Belgium
E-mail: jan.schrooten@mtm.kuleuven.be

Abstract

Affinity probe capillary electrophoresis (APCE) holds promise to be a powerful tool in diagnostic applications that rely on the selective and sensitive detection of proteins. Aptamers can serve to unlock the full potential of the technology since their use opens up possibilities to unite the immuno-sensing and separating strength of capillary electrophoresis with the signal enhancement power of nucleic acid amplification. To enable routine use of aptamer APCE, careful selection of the experimental conditions is crucial. Here it is shown how customization and careful consideration of the experimental parameters can significantly improve assay performance.

Graphical abstract: Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis

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Article information

DOI
https://doi.org/10.1039/C1AY05124E
Article type
Technical Note
Submitted
06 Mar 2011
Accepted
09 Jun 2011
First published
18 Jul 2011

Anal. Methods, 2011,3, 2156-2159

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Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis

K. P. F. Janssen, K. Knez, J. Pollet, S. J. Roberts, J. Schrooten and J. Lammertyn, Anal. Methods, 2011, 3, 2156 DOI: 10.1039/C1AY05124E

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