Issue 9, 2010
From the journal:

Analytical Methods

Qualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs from different species by HPLC

Michael W. Schäffer,a   Somdutta Sinha Roy,a   Shyamali Mukherjee,a   Donatus Nohr,b   Michael Wolter,b   Hans K. Biesalski,b   David E. Ongc  and  Salil K. Das*a  

* Corresponding authors

a Meharry Medical College, Dept. of Biochemistry and Cancer Biology, 1005, Dr D.B. Todd, Jr. Blvd., Nashville, U.S.A
E-mail: sdas@mmc.edu
Fax: +1 615- 327- 6442
Tel: +1 615-327-6988

b Department of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstraße 30, Stuttgart, Germany

c Department of Biochemistry, Vanderbilt University Medical Center, Nashville, U.S.A

Abstract

We report the validation of a reversed-phase gradient HPLC method allowing simultaneous quantification of retinol, retinyl esters, tocopherols and selected carotenoids in lung, liver and plasma of mouse, rat and guinea pig (gp) using a diode array detector. A significant species difference was observed regarding the distribution of retinol and retinyl esters. The levels of total retinol in lung, liver and plasma were in the following order: mouse ≫ rat > gp; rat >mouse > gp; and gp ≫ rat > mouse, respectively. Furthermore, comparison studies revealed similarities between the vitamin A profiles of human and gp lung samples.

Graphical abstract: Qualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs from different species by HPLC

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Article information

DOI
https://doi.org/10.1039/C0AY00288G
Article type
Paper
Submitted
03 May 2010
Accepted
11 Jun 2010
First published
30 Jul 2010

Anal. Methods, 2010,2, 1320-1332

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Qualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs from different species by HPLC

M. W. Schäffer, S. S. Roy, S. Mukherjee, D. Nohr, M. Wolter, H. K. Biesalski, D. E. Ong and S. K. Das, Anal. Methods, 2010, 2, 1320 DOI: 10.1039/C0AY00288G

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