Issue 9, 2010
From the journal:

Analytical Methods

An immobilized dehydrohalogenase based potentiometric biosensor for the detection of chlorinated pesticides†

Mahadevu Rajashekara Murthy,a   Iychettira Machaiah Mandappa,‡a   Rangachary Latha,a   Aaydha Chidambara Vinayaka,a   Munna Singh Thakur*a  and  Haravey Krishnan Manonmania  

* Corresponding authors

a Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, India
E-mail: msthakur@cftri.res.in, msthakur@yahoo.com
Fax: +91 821 2517233
Tel: +91 821 2515792

Abstract

Among the organochlorine pesticides, DDT and HCH are the major cause for food and environmental contamination. To detect them effectively in water samples an immobilized dehydrohalogenase based potentiometric biosensor was developed. The chloride ion released as a result of dehalogenation by immobilized dehydrohalogenase during the degradation of DDT and HCH, respectively, was detected using an ion selective electrode. The voltage response had a direct linear relationship with the concentration of chloride (Cl−) released by DDT and HCH, respectively. The immobilization was advantageous in improving the enzyme property and could be used repeatedly without losing activity.

Graphical abstract: An immobilized dehydrohalogenase based potentiometric biosensor for the detection of chlorinated pesticides

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Article information

DOI
https://doi.org/10.1039/C0AY00272K
Article type
Paper
Submitted
29 Apr 2010
Accepted
10 Jul 2010
First published
10 Aug 2010

Anal. Methods, 2010,2, 1355-1359

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An immobilized dehydrohalogenase based potentiometric biosensor for the detection of chlorinated pesticides

M. R. Murthy, I. M. Mandappa, R. Latha, A. C. Vinayaka, M. S. Thakur and H. K. Manonmani, Anal. Methods, 2010, 2, 1355 DOI: 10.1039/C0AY00272K

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