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Issue 8, 2010
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An amperometric polyphenol biosensor based on polyvinyl chloride membrane

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Abstract

A method is described for construction of a polyphenol biosensor employing polyvinylchloride membrane bound banana fruit polyphenol oxidase. The biosensor showed optimum response within 30 s, when operated at 0.4 V in 0.1 M sodium phosphate buffer, pH 7.5 and 35 °C. The detection limit of the method was 7.5 × 10−7 M. The analytical recovery of added L-DOPA was 93.50%. Within batch and between batch coefficients of variation were < 0.82% and < 1.65% respectively. The sensor was employed for amperometric determination of polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 200 times over 60 days, when stored at 4 °C.

Graphical abstract: An amperometric polyphenol biosensor based on polyvinyl chloride membrane

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Publication details

The article was received on 10 Mar 2010, accepted on 10 May 2010 and first published on 04 Jun 2010


Article type: Paper
DOI: 10.1039/C0AY00165A
Citation: Anal. Methods, 2010,2, 1106-1111
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    An amperometric polyphenol biosensor based on polyvinyl chloride membrane

    S. Chawla, J. Narang and C. S. Pundir, Anal. Methods, 2010, 2, 1106
    DOI: 10.1039/C0AY00165A

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