Issue 11, 2010
From the journal:

Analytical Methods

Imaging of living mammalian retina ex vivo by confocal laser scanning microscopy

Daniela Calzia,a   Paolo Bianchini,b   Silvia Ravera,a   Angela Bachi,c   Giovanni Candiano,d   Alberto Diasprob  and  Isabella Panfoli*a  

* Corresponding authors

a Department of Biology, University of Genoa, Genova, Italy
E-mail: dcalzia@gmail.com, silvia.ravera@gmail.com, Isabella.Panfoli@unige.it

b Italian Inst. of Technology-IIT, Via Morego 30, 16163 Genova, Italy
E-mail: paolo.bianchini@iit.it, alberto.diaspro@iit.it

c DIBIT-San Raffaele Scientific Institute, Via Olgettina 60, Milano, Italy
E-mail: bachi.angela@hsr.it

d Laboratory on Pathophysiology of Uraemia, Genova, Italy
E-mail: giovannicandiano@ospedale-gaslini.ge.it

Abstract

The present paper describes a new procedure for labelling living retinas, ex vivo, with fluorescent dyes, in order to image them by microscopy techniques by a procedure that maintains the physiological features of the tissue and its association with the retinal pigmented epithelium (RPE).

Graphical abstract: Imaging of living mammalian retina ex vivo by confocal laser scanning microscopy

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Article information

DOI
https://doi.org/10.1039/C0AY00120A
Article type
Technical Note
Submitted
18 Feb 2010
Accepted
27 Aug 2010
First published
29 Sep 2010

Anal. Methods, 2010,2, 1816-1818

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Imaging of living mammalian retina ex vivo by confocal laser scanning microscopy

D. Calzia, P. Bianchini, S. Ravera, A. Bachi, G. Candiano, A. Diaspro and I. Panfoli, Anal. Methods, 2010, 2, 1816 DOI: 10.1039/C0AY00120A

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