Colorimetric sensor for cimetidine detection in human urine based on d-xylose protected gold nanoparticles

Abstract

Herein, a simple, novel, and rapid colorimetric sensor for cimetidine (Cim) detection based on D-xylose protected gold nanoparticles (D-x@AuNPs) has been developed for the first time. The D-x@AuNPs were characterized by UV-vis, TEM and FT-IR techniques. Cimetidine causes the aggregation of D-x@AuNPs due to the formation of a strong covalent Au–N bond and electrostatic binding. As the cimetidine concentration increased, the color of the solutions gradually changed from wine-red to blue, and the large absorption band shifted from 533 to 680 nm upon D-x@AuNP aggregation. The effects of different experimental parameters were investigated. A comparative study on the detection of Cim using citrated capped AuNPs and D-x@AuNPs was presented. Under optimum conditions, the UV-vis spectra showed that the absorption ratio (A₆₈₀/A₅₃₃) increased linearly with the concentration of cimetidine in the range of 7 × 10⁻⁸ to 3 × 10⁻⁶ M with a correlation coefficient of 0.9956 and a limit of detection of 1 × 10⁻⁸ M without needing any complicated instruments. The selectivity of the D-x@AuNP detection system for cimetidine was excellent when compared with other ions and analytes. Due to their rapid and visible color changes, and their remarkable selectivity, the D-x@AuNPs synthesized in this study were suitable and could be applied to the detection of cimetidine in human urine.