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Issue 15, 2015
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FT-IR imaging for quantitative determination of liver fat content in non-alcoholic fatty liver

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Abstract

In this work we apply FT-IR imaging of large areas of liver tissue cross-section samples (∼5 cm × 5 cm) for quantitative assessment of steatosis in murine model of Non-Alcoholic Fatty Liver (NAFLD). We quantified the area of liver tissue occupied by lipid droplets (LDs) by FT-IR imaging and Oil Red O (ORO) staining for comparison. Two alternative FT-IR based approaches are presented. The first, straightforward method, was based on average spectra from tissues and provided values of the fat content by using a PLS regression model and the reference method. The second one – the chemometric-based method – enabled us to determine the values of the fat content, independently of the reference method by means of k-means cluster (KMC) analysis. In summary, FT-IR images of large size liver sections may prove to be useful for quantifying liver steatosis without the need of tissue staining.

Graphical abstract: FT-IR imaging for quantitative determination of liver fat content in non-alcoholic fatty liver

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Publication details

The article was received on 16 Apr 2015, accepted on 15 May 2015 and first published on 18 May 2015


Article type: Communication
DOI: 10.1039/C5AN00737B
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 4997-5002
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    FT-IR imaging for quantitative determination of liver fat content in non-alcoholic fatty liver

    K. Kochan, E. Maslak, S. Chlopicki and M. Baranska, Analyst, 2015, 140, 4997
    DOI: 10.1039/C5AN00737B

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