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Issue 9, 2015
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Distyrylbenzene-aldehydes: identification of proteins in water

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Abstract

Three different, water soluble, aldehyde-appended distyrylbenzene (DSB) derivatives were prepared. Their interaction with different albumin variants (human, porcine, bovine, lactalbumin, ovalbumin) was investigated (pH 11). All three fluorophores exhibit graded, protein-dependent fluorescence turn-on at slightly differing wavelengths. Linear discriminant analysis (LDA) differentiated all of the investigated albumins and was used to discern commercially available protein shakes. The three DSB derivatives barely react with the constituting amino acids but cysteine. In the proteins significant fluorescence signals are generated, probably due to a combination of imine/N,S-aminal formation and hydrophobic interactions between the DSBs and the proteins.

Graphical abstract: Distyrylbenzene-aldehydes: identification of proteins in water

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Publication details

The article was received on 23 Jan 2015, accepted on 12 Mar 2015 and first published on 12 Mar 2015


Article type: Paper
DOI: 10.1039/C5AN00155B
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 3136-3142
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    Distyrylbenzene-aldehydes: identification of proteins in water

    J. Kumpf, J. Freudenberg and U. H. F. Bunz, Analyst, 2015, 140, 3136
    DOI: 10.1039/C5AN00155B

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