Issue 21, 2014

Label free detection of specific protein binding using a microwave sensor

Abstract

The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.

Graphical abstract: Label free detection of specific protein binding using a microwave sensor

Supplementary files

Article information

Article type
Communication
Submitted
19 May 2014
Accepted
04 Aug 2014
First published
04 Aug 2014

Analyst, 2014,139, 5335-5338

Author version available

Label free detection of specific protein binding using a microwave sensor

M. Salazar-Alvarez, O. Korostynska, A. Mason, A. Al-Shamma'a, J. C. Cooney, E. Magner and S. A. M. Tofail, Analyst, 2014, 139, 5335 DOI: 10.1039/C4AN00909F

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