Issue 1, 2014
From the journal:

Analyst

Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores†

Tucker J. Carroccia  and  Aaron A. Hoskins*a  

* Corresponding authors

a Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706, USA
E-mail: ahoskins@wisc.edu
Fax: +1-608-262-3453
Tel: +1-608-890-3101

Abstract

We have developed new protein-based tags for use in imaging of RNAs in living cells. These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range.

Graphical abstract: Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores

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Article information

DOI
https://doi.org/10.1039/C3AN01550E
Article type
Communication
Submitted
14 Aug 2013
Accepted
27 Oct 2013
First published
28 Oct 2013

Analyst, 2014,139, 44-47

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Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores

T. J. Carrocci and A. A. Hoskins, Analyst, 2014, 139, 44 DOI: 10.1039/C3AN01550E

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