Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

Terence G. Henares; Yuta Uenoyama; Yuto Nogawa; Ken Ikegami; Daniel Citterio; Koji Suzuki; Shun-ichi Funano; Kenji Sueyoshi; Tatsuro Endo; Hideaki Hisamoto

doi:10.1039/C3AN00098B

Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)†

Terence G. Henares,‡^a Yuta Uenoyama,‡^a Yuto Nogawa,^a Ken Ikegami,^b Daniel Citterio,^b Koji Suzuki,^b Shun-ichi Funano,^a Kenji Sueyoshi,^a Tatsuro Endo^a and Hideaki Hisamoto*^a

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^a Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai City, Osaka 599-8531, Japan
E-mail: hisamoto@chem.osakafu-u.ac.jp
Fax: +81-72-254-9284
Tel: +81-72-254-9285

^b Faculty of Science and Technology, Keio University, Kanagawa, Japan

Abstract

This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1–2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

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Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)†

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Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

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