Issue 19, 2012

Sample preparation and direct electrospray ionization on a tip column for rapid mass spectrometry analysis of complex samples

Abstract

A handheld pipette tip column electrospray ionization source (PTC-ESI source) was developed for rapid mass spectrometry analysis at ambient pressure. The PTC-ESI source was made up of three main component parts including a micro DC high voltage (HV) power supply, a micropipette and a disposable micropipette tip filled with a plug of adsorbent. A DC high voltage was applied to the sharp point of the micropipette tip column to induce electrospray ionization. The PTC-ESI source was successfully used for direct analysis of basic organic compounds, organic acids and peptides in a simple matrix. In the case of complex samples, micro-extraction based on the adsorbent phase filled in the pipette tip was used to remove impurities and concentrate target analytes prior to ionization. The eluting solution was not pipetted out, but directly dispersed in the form of electrospray from the pipette tip for ionization. The effectiveness of the PTC-ESI source has been further demonstrated by fast analysis of therapeutic compounds and endogenous bioactive chemicals in complex biological samples.

Graphical abstract: Sample preparation and direct electrospray ionization on a tip column for rapid mass spectrometry analysis of complex samples

Article information

Article type
Paper
Submitted
24 Jun 2012
Accepted
03 Aug 2012
First published
06 Aug 2012

Analyst, 2012,137, 4593-4597

Sample preparation and direct electrospray ionization on a tip column for rapid mass spectrometry analysis of complex samples

Y. Huang, J. You, B. Yuan and Y. Feng, Analyst, 2012, 137, 4593 DOI: 10.1039/C2AN35856E

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