Issue 9, 2012

A promising dehydrogenase-based bioanode for a glucose biosensor and glucose/O2 biofuel cell

Abstract

A new type of dehydrogenase-based amperometric glucose biosensor was constructed using glucose dehydrogenase (GDH) which was immobilized on the edge-plane pyrolytic graphite (EPPG) electrode modified with poly(phenosafranin)-functionalized single-walled carbon nanotubes (PPS-SWCNTs). The PPS-SWCNT-modified EPPG electrode was prepared by electropolymerization of phenosafranin on the EPPG electrode which had been previously coated with SWCNTs. The performance of the GDH/PPS-SWCNT/EPPG bioanode was evaluated using cyclic voltammetry and amperometry in the presence of glucose. The GDH/PPS-SWCNT/EPPG electrode possesses promising characteristics as a glucose sensor: a wide linear dynamic range of 50 to 700 μM, low detection limit of 0.3 μM, fast response time (1–2 s), high sensitivity (96.5 μA cm−2 mM−1), and anti-interference and anti-fouling abilities. Moreover, the performance of the GDH/PPS-SWCNT/EPPG bioanode was tested in a glucose/O2 biofuel cell. The maximum power density delivered by the assembled glucose/O2 biofuel cell could reach 64.0 μW cm−2 at a cell voltage of 0.3 V with 40 mM glucose.

Graphical abstract: A promising dehydrogenase-based bioanode for a glucose biosensor and glucose/O2 biofuel cell

Article information

Article type
Paper
Submitted
17 Oct 2011
Accepted
31 Jan 2012
First published
14 Mar 2012

Analyst, 2012,137, 2233-2238

A promising dehydrogenase-based bioanode for a glucose biosensor and glucose/O2 biofuel cell

F. S. Saleh, L. Mao and T. Ohsaka, Analyst, 2012, 137, 2233 DOI: 10.1039/C2AN15971F

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