Issue 22, 2011
From the journal:

Analyst

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection†

Ana Knežević,‡a   Jonathan Bones,‡b   Stjepan Krešimir Kračun,c   Olga Gornik,d   Pauline M. Ruddb  and  Gordan Lauc*ad  

* Corresponding authors

a Genos Ltd, Laboratory for Glycobiology, Planinska 1, Zagreb, Croatia
E-mail: glauc@pharma.hr

b NIBRT Dublin-Oxford Glycobiology Laboratory, National Institute for Bioprocessing Research and Training, Dublin 4, Ireland

c Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark

d Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, University of Zagreb, Croatia

Abstract

A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

Graphical abstract: High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

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Article information

DOI
https://doi.org/10.1039/C1AN15684E
Article type
Communication
Submitted
01 Aug 2011
Accepted
14 Sep 2011
First published
29 Sep 2011

Analyst, 2011,136, 4670-4673

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High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

A. Knežević, J. Bones, S. K. Kračun, O. Gornik, P. M. Rudd and G. Lauc, Analyst, 2011, 136, 4670 DOI: 10.1039/C1AN15684E

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