Issue 22, 2011

A microfluidic device based on a screen-printed carbon electrode with electrodeposited gold nanoparticles for the detection of IgG anti-Trypanosoma cruziantibodies

Abstract

In this article we report the development of an integrated microfluidic system coupled to a screen-printed carbon electrode (SPCE) applied to the quantitative determination of IgG specific antibodies present in serum samples of patients that suffer from Chagas disease. This relevant parasitic infection caused by the hemoflagellate protozoan Trypanosoma cruzi represents a major public health concern in Latin America. In order to perform the detection of mentioned antibodies, SPCE coupled to a microfluidic device was modified by electrodeposition of gold nanoparticles (AuNPs) and functionalized with Trypanosoma cruziproteins from epimastigote membranes. The developed microfluidic immunosensor with immobilized T. cruziproteins on the SPCE surface was successfully applied in the detection of specific IgG anti-T. cruziantibodies, which were allowed to react immunologically with immobilized T. cruziantigen. After that, labelled antibodies were quantified through the addition of horseradish peroxidase (HRP) enzyme-labeled secondary antibodies specific to human IgG, using 4-tert-butylcatechol (4-TBC) as enzymatic mediator. HRP in the presence of hydrogen peroxide (H2O2) catalyzes the oxidation of 4-TBC whose back electrochemical reduction was detected on a modified electrode at −100 mV. The calculated detection limit for electrochemical detection was 3.065 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6.95%.

Graphical abstract: A microfluidic device based on a screen-printed carbon electrode with electrodeposited gold nanoparticles for the detection of IgG anti-Trypanosoma cruziantibodies

Article information

Article type
Paper
Submitted
08 Jul 2011
Accepted
13 Sep 2011
First published
10 Oct 2011

Analyst, 2011,136, 4745-4751

A microfluidic device based on a screen-printed carbon electrode with electrodeposited gold nanoparticles for the detection of IgG anti-Trypanosoma cruziantibodies

S. V. Pereira, F. A. Bertolino, M. A. Fernández-Baldo, G. A. Messina, E. Salinas, M. I. Sanz and J. Raba, Analyst, 2011, 136, 4745 DOI: 10.1039/C1AN15569E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements