Detection of hydrogen peroxide in rainwater based on Mg-Al-carbonate layered double hydroxides-catalyzed luminol chemiluminescence

Abstract

Using a green catalyst of luminol chemiluminescence (CL), Mg-Al-carbonate layered double hydroxides (denoted as Mg-Al-CO₃ LDHs), a novel, sensitive and rapid CL method was developed for the determination of hydrogen peroxide (H₂O₂). The corresponding linear regression equation was established in the range of 0.05–10 μM for H₂O₂. The detection limit (S/N = 3) is 0.02 μM and the relative standard deviation (RSD) for nine repeated measurements of 1.0 μM H₂O₂ was 2.9%. This proposed method has been successfully applied to detect H₂O₂ in rainwater samples with good accuracy and precision. The novel methodology is expected to provide a general protocol for the determination of H₂O₂ as well as for numerous other oxidase-based reactions giving H₂O₂ as a product (e.g., glucose).