Jump to main content
Jump to site search

Issue 15, 2011
Previous Article Next Article

Direct detection of fatty acid ethyl esters using low temperature plasma (LTP) ambient ionization mass spectrometry for rapid bacterial differentiation

Author affiliations

Abstract

Low temperature plasma mass spectrometry (LTP-MS) was employed to detect fatty acid ethyl esters (FAEE) from bacterial samples directly. Positive ion mode FAEE mass spectrometric profiles of sixteen different bacterial samples were obtained without extraction or other sample preparation. In the range m/z 200–300, LTP mass spectra show highly reproducible and characteristic patterns. To identify the FAEE's associated with the characteristic peaks, accurate masses were recorded in the full scan mode using an LTQ/Orbitrap instrument, and tandem mass spectrometry was performed. Data were examined by principal component analysis (PCA) to determine the degree of differentiation possible amongst different bacterial species. Gram-positive and gram-negative bacteria are readily distinguished, and 11 out of 13 Salmonella strains show distinctive patterns. Growth media effects are observed but do not interfere with species recognition based on the PCA results.

Graphical abstract: Direct detection of fatty acid ethyl esters using low temperature plasma (LTP) ambient ionization mass spectrometry for rapid bacterial differentiation

Back to tab navigation

Publication details

The article was received on 24 Nov 2010, accepted on 10 Jun 2011 and first published on 27 Jun 2011


Article type: Paper
DOI: 10.1039/C0AN00940G
Citation: Analyst, 2011,136, 3091-3097
  •   Request permissions

    Direct detection of fatty acid ethyl esters using low temperature plasma (LTP) ambient ionization mass spectrometry for rapid bacterial differentiation

    J. I. Zhang, A. B. Costa, W. A. Tao and R. G. Cooks, Analyst, 2011, 136, 3091
    DOI: 10.1039/C0AN00940G

Search articles by author

Spotlight

Advertisements