Issue 1, 2010
From the journal:

Analyst

Analytical protocol for identification of BMAA and DAB in biological samples

Zdeněk Spáčil,†bc   Johan Eriksson,†*a   Sara Jonasson,a   Ulla Rasmussen,a   Leopold L. Ilag*b  and  Birgitta Bergmana  

* Corresponding authors

a Department of Botany, Stockholm University, Stockholm, Sweden
E-mail: johan.eriksson@botan.su.se
Fax: +46 (0)8 165525
Tel: +46 (0)8 163793

b Department of Analytical Chemistry, Stockholm University, Stockholm, Sweden
E-mail: leopold.ilag@anchem.su.se
Fax: +46 (0)8 156391
Tel: +46 (0)8 162435

c Faculty of Pharmacy, Charles University in Prague, Czech Republic

Abstract

β-N-methylamino-L-alanine (BMAA) is a non-protein amino acid, thought to be inflicting neurodegenerative diseases related to ALS/PDC in human beings. Due to conflicting data concerning the presence of BMAA in various biological matrixes, we present a robust and sensitive method for high confidence identification of BMAA after derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The efficient sample pretreatment in combination with LC-MS/MS SRM enables chromatographic separation of BMAA from the isomer 2,3-diaminobutyric acid (DAB). The method is applicable for selective BMAA/DAB detection in various biological samples ranging from a prokaryotic cyanobacterium to eukaryotic fish.

Graphical abstract: Analytical protocol for identification of BMAA and DAB in biological samples

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Article information

DOI
https://doi.org/10.1039/B921048B
Article type
Paper
Submitted
07 Oct 2009
Accepted
16 Nov 2009
First published
26 Nov 2009

Analyst, 2010,135, 127-132

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Analytical protocol for identification of BMAA and DAB in biological samples

Z. Spáčil, J. Eriksson, S. Jonasson, U. Rasmussen, L. L. Ilag and B. Bergman, Analyst, 2010, 135, 127 DOI: 10.1039/B921048B

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