Issue 7, 2009
From the journal:

Analyst

Separation of oligonucleotides with single-base mutation by capillary electrophoresis using specific interaction of metal ion with nucleotide

Toru Takahashi,*a   Takao Sakurai,a   Koichiro Hiratab  and  Hitoshi Hoshinoa  

* Corresponding authors

a Division of Environmentally Benign Systems, Graduate School of Environmental Studies, Tohoku University, 20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
E-mail: toru@analchem.che.tohoku.ac.jp
Fax: +81-22-795-7223
Tel: +81-22-795-7224

b Department of Molecular Engineering, Faculty of Engineering, Tohoku University, 20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
Fax: +81-22-795-7223
Tel: +81-22-795-7224

Abstract

A unique tactic for the separation of single-base sequential isomers of oligomeric single-stranded DNA by a CE separation system employing the specific interaction of metal ion with nucleotide was demonstrated, enabling the separation of the mixture of a 12-mer oligonucleotide and its single-base mutants, as well as their positional isomers.

Graphical abstract: Separation of oligonucleotides with single-base mutation by capillary electrophoresis using specific interaction of metal ion with nucleotide

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Article information

DOI
https://doi.org/10.1039/B903440D
Article type
Communication
Submitted
18 Feb 2009
Accepted
21 Apr 2009
First published
28 Apr 2009

Analyst, 2009,134, 1299-1301

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Separation of oligonucleotides with single-base mutation by capillary electrophoresis using specific interaction of metal ion with nucleotide

T. Takahashi, T. Sakurai, K. Hirata and H. Hoshino, Analyst, 2009, 134, 1299 DOI: 10.1039/B903440D

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