Issue 10, 2008
From the journal:

Analyst

A near-infrared fluorescent probe for lipid hydroperoxides in living cells

Ping Li,a   Bo Tang,*a   Yanlong Xing,a   Puming Li,a   Guiwen Yangb  and  Liang Zhangb  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, P. R. China
E-mail: tangb@sdnu.edu.cn
Fax: +86-531-86180017
Tel: +86-531-86180010

b College of Life Science, Shandong Normal University, Jinan 250014, P. R. China

Abstract

An NIR (near-infrared) fluorescent probe TCP (tricarbocyanine diphenylphosphine) including a non-conjugated ‘pre-tricarbocyanine’ was designed and synthesized for visualizing lipid hydroperoxides (ROOH) in living cells. The excitation and emission spectra of tricarbocyanine in the NIR region can effectively avoid background fluorescence interference in biological systems. The probe exhibited a rapid fluorescence response to ROOH and high selectivity for ROOH over other ROS (reactive oxygen species) and some biological compounds, and the limit of detection was 38 pM. In addition, the probe was stable, and less cytotoxic, which indicated that it has potential application in detecting lipid hydroperoxides in living biological systems.

Graphical abstract: A near-infrared fluorescent probe for lipid hydroperoxides in living cells

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Article information

DOI
https://doi.org/10.1039/B802836B
Article type
Paper
Submitted
19 Feb 2008
Accepted
21 Apr 2008
First published
15 May 2008

Analyst, 2008,133, 1409-1415

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A near-infrared fluorescent probe for lipid hydroperoxides in living cells

P. Li, B. Tang, Y. Xing, P. Li, G. Yang and L. Zhang, Analyst, 2008, 133, 1409 DOI: 10.1039/B802836B

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