Issue 5, 2008

A hydrodynamic approach to the measurement of the permeability of small molecules across artificial membranes

Abstract

An in situ analytical approach to the measurement of supported liquid membrane permeability is reported. The method consists of a spectrophotometric method to measure transport through a membrane-supported lipid solution, using a rotating-diffusion cell configuration to overcome limits arising from transport through the aqueous solution boundary layer in stationary systems. Rotation frequencies are almost two orders of magnitude higher than those employed previously for rotating-diffusion studies of membrane transport. The method is illustrated with the transport of warfarin [1-(4′-hydroxy-3′-coumarinyl)-1-phenyl-3-butanone]. The use of the rotating-diffusion approach permits accurate calculation of the aqueous phase boundary layer thickness, which has hitherto been treated as an adjustable parameter in studies of membrane permeability. Further, it is shown that the analyte diffusion coefficient can be determined readily using liquid–liquid electrochemistry.

Graphical abstract: A hydrodynamic approach to the measurement of the permeability of small molecules across artificial membranes

Article information

Article type
Paper
Submitted
19 Dec 2007
Accepted
12 Feb 2008
First published
14 Mar 2008

Analyst, 2008,133, 655-659

A hydrodynamic approach to the measurement of the permeability of small molecules across artificial membranes

B. Molloy, K. Y. Tam, J. Matthew Wood and R. A. W. Dryfe, Analyst, 2008, 133, 655 DOI: 10.1039/B719634B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements