Issue 5, 2008
From the journal:

Analyst

Antibody–gold quantum dot–PAMAM dendrimer complex as an immunoglobulin immunoassay†

Robert C. Triulzi,a   Miodrag Micic,*b   Jhony Orbulescu,a   Silvia Giordani,c   Bill Muellerd  and  Roger M. Leblanc*a  

* Corresponding authors

a University of Miami, Department of Chemistry, 1301 Memorial Drive, Coral Gables, FL 33146, USA
E-mail: rml@miami.edu
Fax: +1-305-284-6367
Tel: +1-305-284-2194

b MP Biomedicals LLC, 3 Hutton Center, Suite 100, Santa Ana, CA 92707, USA
E-mail: mmicic@mpbio.com
Fax: +1-949-859-5095
Tel: +1-949-833-2500 ext. 264

c Trinity College - Dublin, Dublin-2, Ireland

d Veeco Instruments Inc, 112 Robin Hill Road, Santa Barbara, CA 93117, USA

Abstract

Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.

Graphical abstract: Antibody–gold quantum dot–PAMAM dendrimer complex as an immunoglobulin immunoassay

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Article information

DOI
https://doi.org/10.1039/B718730K
Article type
Paper
Submitted
05 Dec 2007
Accepted
29 Feb 2008
First published
20 Mar 2008

Analyst, 2008,133, 667-672

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Antibody–gold quantum dot–PAMAM dendrimer complex as an immunoglobulin immunoassay

R. C. Triulzi, M. Micic, J. Orbulescu, S. Giordani, B. Mueller and R. M. Leblanc, Analyst, 2008, 133, 667 DOI: 10.1039/B718730K

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