Using coupled monolithic rods for ultra-high peak capacity LC and LC–MS under normal LC operating pressures

Jonathan Bones; Cepta Duffy; Mirek Macka; Brett Paull

doi:10.1039/B714807K

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Using coupled monolithic rods for ultra-high peak capacity LC and LC–MS under normal LC operating pressures†‡

Jonathan Bones,^a Cepta Duffy,^a Mirek Macka^b and Brett Paull*^ab

Author affiliations

* Corresponding authors

^a Centre for Bioanalytical Science, School of Chemical Sciences, Dublin City University, Dublin, Ireland

^b National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin, Ireland
E-mail: brett.paull@dcu.ie
Fax: +353 1 7005503
Tel: +353 1 7005060

Abstract

Up to ten individual reversed-phase monolithic LC columns were efficiently coupled to equal a total column length of 1 m. The column generated up to 90 000 theoretical plates under optimum conditions with a peak capacity under gradient conditions of 244 peaks h⁻¹ at a total column backpressure of just 140 bar, directly coupled to ESI-MS.

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Supplementary files

Fig. S1 for van Deemter curves for acetophenone on each column length investigated; Fig. S2 for graph and accompanying data for P _c versus column length; Fig. S3 for graph showing the relationship between column pressure drop and plate generation for butylbenzene; Fig. S4 for comparison of 1 m monolithic phase and 1.8 μm particle-packed phase for protein digest separation under similar gradient conditions at equal column pressure drops; Table S1 for individual column relative efficiencies and pressure drop data PDF (432K)

Article information

DOI: https://doi.org/10.1039/B714807K
Article type: Communication
Submitted: 26 Sep 2007
Accepted: 06 Dec 2007
First published: 17 Dec 2007

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Analyst, 2008,133, 180-183

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Using coupled monolithic rods for ultra-high peak capacity LC and LC–MS under normal LC operating pressures

J. Bones, C. Duffy, M. Macka and B. Paull, Analyst, 2008, 133, 180 DOI: 10.1039/B714807K

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Using coupled monolithic rods for ultra-high peak capacity LC and LC–MS under normal LC operating pressures†‡

Abstract

Supplementary files

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Using coupled monolithic rods for ultra-high peak capacity LC and LC–MS under normal LC operating pressures

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