Identification of selenomethionine in selenized yeast using two-dimensional liquid chromatography-mass spectrometry based proteomic analysis

Abstract

Selenium-enriched yeast has been commonly used as a nutritional supplement. Here we describe a protocol used to investigate the metabolic fate of inorganic selenium in yeast. We provide definitive, mass spectrometry based evidence for the non-specific incorporation of selenomethionine in the yeast proteome involving the replacement of about 30% of all methionine with selenomethionine.