Issue 10, 2003

Determination of trace concentrations of dissolved nitric oxide in a biological buffer

Abstract

A new real-time method for measuring a trace concentration of nitric oxide (NO) in a complex matrix routinely used in pharmacological studies of its bioactivity is described. NO was quantified as a gas by chemiluminescence after extraction from a continuous liquid sample flow with a limit of detection of 0.042 nmol dm−3 at a signal to noise ratio of 3. Theories to calculate the concentration of NO in the liquid sample flow from a direct measurement of NO in the extraction carrier gas are presented. The efficiency of extraction is determined by a stopflow experiment. An example is presented of the measurement of the steady-state concentrations of NO in Krebs-bicarbonate buffer at pH 7.4 and 37 °C when its liquid surface is sequentially exposed to gases containing various concentrations of NO in O2 plus CO2.

Article information

Article type
Paper
Submitted
22 Aug 2003
Accepted
28 Aug 2003
First published
15 Sep 2003

Analyst, 2003,128, 1281-1285

Determination of trace concentrations of dissolved nitric oxide in a biological buffer

E. A. G. Demoncheaux, P. J. Foster, C. D. Borland, A. P. L. Smith, T. W. Higenbottam and M. B. Davies, Analyst, 2003, 128, 1281 DOI: 10.1039/B310188F

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